Tumor Tissue

Transform your cancer research with the innovative inCHIPit™ and comPLATE™ combination. Create a fully tumor-derived model that mimics the original tumor and get one step closer to unlocking the underlying mechanisms of cancer.

ExVivo Breast Cancer

Get direct access to your tumor tissue from the top and observe the progress of your research with ease through the optically transparent window on the bottom. In addition, with the ability to line the microfluidic channel with endothelial cells, you can create a perfusable 3D blood vessel and stimulate the growth of your tumor model.

This not only gives you a more accurate representation of the original tumor, but it also allows you to test the impact of chemotherapies and other substances on the growth and spread of the cancer.

The inCHIPit™ and comPLATE™ are the perfect tools for a more in-depth understanding of the complexities of cancer! 

The proof is in the results

In collaboration with the Erasmus Medical Center in Rotterdam, Netherlands, we conduced a study where the inCHIPit™ and comPLATE™ were used to culture and test breast- and prostate cancer tissue, and obtained remarkable results on drug response. Check out the full study from Chakrabarty et al. 2022 here.

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Cancer treatment faces major challenges in defining the optimal therapy for each individual patient. Although there have been several strategies employed for this, to date, none have been able to reliably predict individual response. Therefore, ex vivo assays that can predict this response would strongly aid selection of the most effective treatment for each patient tumor(s).

In this article, we show how tumor tissue was cultured in the Bi/ond organ-on-chip system, consisting of the inCHIPit™ 3C with the comPLATE™, here referred to as cancer-on-chip (CoC) system. Breast PDX tumor and prostate PDX tumor PC82 slices were either cultured in 6-well standard plates (referred to as ex vivo culture) or in the Bi/ond CoC platform in duplicates.

Scientist removed tissue slices from the chip at either day 7 or day 14 and fixed them in formalin and embedded them in paraffin for microscopic analysis.  First, the condition of the cells in the 6-well ex vivo culture was compared with that of the cells in the CoC platform after 7 and 14 days of culturing.

The obtained proliferation and cell death data indicate that the CoC platform provides better culture conditions for extended culture times  (beyond 7 days) of tumor tissue slices than the ex vivo system. There are already subtle differences between both culture methods even after 7 days, as shown in the figure on the right.

Tumor Graph 7 days

Drug response

Next, researchers treated breast tumor slices for 3 days with Cisplatin. Tissue cultured in the CoC platform showed a decrease in replicating (s-phase) cells and significant increase in apoptotic cells as compared to ex vivo culturing. In the figure on the right you can see EdU-positive (replicating) cells and TUNEL-positive (apoptotic) cells at day 0 (D0), day 3 (D3) and at day 3 after treatment with Cisplatin (D3Cis). This response to cisplatin treatment correlated well with the known tumor response in vivo as well as in ex vivo cultures.  

A similar response in replicating and apoptotic cells was seen after 7 days of apalutamide exposure of prostate tumor slices. Again, this was comparable to the effect of antiandrogen treatment in in vivo and ex vivo cultures.  

Tumor Drug Response
"We are applying BI/OND devices in therapy response using the breast PDX model. BI/OND microfluidics chips guarantee accurate controlled condition, which is beneficial for long term tumor tissue slice culture. Setting up the Organ-on-Chip technology went very smoothly in our lab.”
Dijk van Gent
Dr. Dik van Gent
Assistant Professor in the department of Molecular Genetics at Erasmus Medical Center

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