APPLICATIONS
BI/OND empowers biologists with advanced hardware solutions for physiological cell culturing.  We developed a platform composed of microfluidic chips and a plate-like holder.
BI/OND platform is versatile as it is qualified for complex 3D tissues (organoids, ex vivo tissue, spheroids, microtissues) cultivation as well as tissue-tissue interface models.
APPLICATIONS
BI/OND empowers biologists with advanced hardware solutions for physiological cell culturing.  We developed a platform composed of microfluidic chips and a plate-like holder.
BI/OND platform is versatile as it is qualified for complex 3D tissues (organoids, ex vivo tissue, spheroids, microtissues) cultivation as well as tissue-tissue interface models.

Ex Vivo tissues

Organoids

Tissue interface

The platform is currently used for organoids, patient-derived samples and tissue-tissue interfaces. Do you want to become an Early Adopter?
The BI/OND CHIP

 

The chip is composed of two compartments connected by a customizable porous membrane. The top compartment, called open well, can accommodate 2D cultures, organoids, patient-derived samples, and many more, providing biologists direct access to the cell culture.
In the bottom compartment, single or multiple micro-channels are located to continuously provide oxygen and nutrients to the tissues. The chip is flexible to provide strain to the tissues like in vivo, and it is optically transparent for imaging.
The BI/OND CHIP
The chip is composed of two compartments connected by a customizable porous membrane. The top compartment, called open well, can accommodate 2D cultures, organoids, patient-derived samples, and many more, providing biologists direct access to the cell culture.
In the bottom compartment, single or multiple micro-channels are located to continuously provide oxygen and nutrients to the tissues. The chip is flexible to provide strain to the tissues like in vivo, and it is optically transparent for imaging.
threechambertryout4
Chip for Ex Vivo Tissues, Organoids, Microtissue: The three channels chip.
The unique open well architecture provides plenty of space for organoids, patient-derived samples, and many more.
The three channels allow refreshing the medium while providing nutrition and oxygenation.
threechambertryout4
Chip for Ex Vivo Tissues, Organoids, Microtissue: The three channels chip.
The unique open well architecture provides plenty of space for organoids, patient-derived samples, and many more.
The three channels allow refreshing the medium while providing nutrition and oxygenation.

Example of a tissue-tissue interface under mechanical stimulation.

Example of an organoid on the open well refreshed by the microfluidic channel underneath.
onechamber
Chip for tissue-tissue interface models: The single channel.
The single channel allows having optimal control of the dynamic flow conditions.
For an optimal tissue-tissue interface, the membrane thickness and porosity can be tuned.
onechamber
Chip for tissue-tissue interface models: The single channel.
The single channel allows having optimal control of the dynamic flow conditions.
For an optimal tissue-tissue interface, the membrane thickness and porosity can be tuned.
The Plate
BI/OND’s plate-like holder it is compact, reusable, requires minimum training and it is fully compatible with any perfusion systems.
One plate with many functionalities thanks to its LEGO-like approach: supports cells and 3D tissue cultures, fluid flow, mechanical stimulation and imaging.
The Plate
BI/OND’s plate-like holder it is compact, reusable, requires minimum training and it is fully compatible with any perfusion systems.
One plate with many functionalities thanks to its LEGO-like approach: supports cells and 3D tissue cultures, fluid flow, mechanical stimulation and imaging.
The plate allows to run up to six cultures in parallel, with the option to perfuse a single compartment (single fluid flow in the microchannel) or both compartments (in the open well as well as in the microfluidic channel) with different flow rates.
The disposable chips can be removed from the holder to collect the cells and for confocal and short distance microscopy.
The plate allows to run up to six cultures in parallel, with the option to perfuse a single compartment (single fluid flow in the microchannel) or both compartments (in the open well as well as in the microfluidic channel) with different flow rates.
The disposable chips can be removed from the holder to collect the cells and for confocal and short distance microscopy.